International Journal of Food Processing Technology  (Volume 3 Issue 1)
 Comparison of Extraction Efficiency of Tanshinones from S. miltiorrhiza by Solvent and Supercritical Carbon Dioxide  International Journal of Food Processing Technology
Pages 1-10

Wei-Der Lee and Bing-Huei Chen

Published: 28 January 2016
Salvia miltiorrhiza (S. miltiorrhiza), also named as Danshen, is a traditional Chinese herbal medicine reported to possess anti-cancer and anti-inflammation activities, which can be attributed to presence of the major functional components tanshinones. The objectives of this study were to develop a high-performance liquid chromatography-mass spectrometry (HPLC-MS) method for determination of tanshinones in S. miltiorrhiza and comparison of extraction efficiency of solvent and supercritical CO2. Results showed that a total of 6 tanshinones including 15,16-dihydrotanshinone I, 1,2,5,6-tetrahydrotanshinone I, cryptotanshinone, tanshinone I, 1,2-dihydrotanshinone I and tanshinone IIA could be separated within 18 min by employing a Metachem ODS-2 C18 column and a gradient mobile phase of 0.1% formic acid solution (A) and acetonitrile (B) with flow rate at 1 mL/min, detection wavelength at 280 nm and column temperature at 25°C. The highest yield of total tanshiones (2869.9 μg/g) extracted by supercritical CO2 was attained at 70°C and 400 bar. For solvent extraction, the highest yield of total tanshinones was obtained by methanol or ethanol, which amounted to 3103.1 μg/g and 3021.6 μg/g, respectively. For future large production of tanshinones from S. miltiorrhiza, ethanol can be adopted to replace methanol and supercritical CO2 amid its safety nature and higher yield than supercritical CO2.
Salvia miltiorrhiza, tanshinones, HPLC-MS, solvent, supercritical CO2.