International Journal of Food Processing Technology  (Volume 2 Issue 1)
 Application of Flow Cytometry and Fluorescence Techniques in Somatic Cell Analysis of Raw Milk  International Journal of Food Processing Technology
Pages 11-16

Merve Pelvan and Sevcan Unluturk

Published: 15 January 2015
 Monitoring the quality and safety of milk requires careful analysis of somatic cell count (SCC). High level of SCC is a signal of diseases such as mastitis. Mastitis can be detected not only by measuring the inflammatory components and pathogens but also evaluating the SCC in milk. In this study, somatic cells are counted with direct microscopy and flow cytometry. For SCC analyses, a centrifugal lipid clearing process is applied. Cleared samples are stained with two fluorescent dyes acridine orange (AO) and ethidium bromide (EtBr), which have different binding mechanism to cell. These two dyes and their different applications are investigated to reveal the effect on somatic cell count. The results are also compared with direct microscopy method. The results of flow cytometry counting method are well correlated with the ones obtained by direct microscopy technique. In conclusion, AO can be an alternative chemical dye for EtBr to be used in enumeration of SCC by flow cytometry technique.
Acridine orange, Ethidium Bromide, Flow cytometry, Raw milk, Somatic cell.