Application of Flow Cytometry and Fluorescence Techniques in Somatic Cell Analysis of Raw Milk

Authors

  • Merve Pelvan Izmir Institute of Technology
  • Sevcan Unluturk Izmir Institute of Technology

Keywords:

orange, Ethidium Bromide, Flow cytometry, Raw milk, Somatic cell.

Abstract

Monitoring the quality and safety of milk requires careful analysis of somatic cell count (SCC). High level of SCC is a signal of diseases such as mastitis. Mastitis can be detected not only by measuring the inflammatory components and pathogens but also evaluating the SCC in milk. In this study, somatic cells are counted with direct microscopy and flow cytometry. For SCC analyses, a centrifugal lipid clearing process is applied. Cleared samples are stained with two fluorescent dyes acridine orange (AO) and ethidium bromide (EtBr), which have different binding mechanism to cell. These two dyes and their different applications are investigated to reveal the effect on somatic cell count. The results are also compared with direct microscopy method. The results of flow cytometry counting method are well correlated with the ones obtained by direct microscopy technique. In conclusion, AO can be an alternative chemical dye for EtBr to be used in enumeration of SCC by flow cytometry technique.

Author Biographies

Merve Pelvan, Izmir Institute of Technology

Food Engineering

Sevcan Unluturk, Izmir Institute of Technology

Food Engineering

Downloads

Published

2015-01-15

Issue

Vol. 2 No. 1 (2015)

Section

Articles

License

Policy for Journals/Articles with Open Access

Authors who publish with this journal agree to the following terms:
    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

  1. Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work

Policy for Journals / Manuscript with Paid Access

Authors who publish with this journal agree to the following terms:
    1. Publisher retain copyright .

  1. Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work .