https://cosmosscholars.com/phms/index.php/wjor/issue/feed World J. of Oncology Research 2019-01-04T13:03:40+00:00 Support Manager support@cosmosscholars.com Open Journal Systems <strong>World Journal of Oncology Research</strong> is a peer-reviewed scientific journal which strengthens the research and educational base of Oncology. The journal provides an active platform for epidemiologists, medical oncologists, radiation oncologists, radiologists, pathologists and researchers all over the world to promote, share, and discuss original scientific latest and outstanding research articles concerned with basic and clinical investigation of cancer, on the cellular, molecular, prevention, diagnosis, therapy and prognosis aspects. https://cosmosscholars.com/phms/index.php/wjor/article/view/887 Inhibition of Ocular Tumor and Endothelial Cell Growth with a TEAD4216 Peptide Fragment 2019-01-04T13:03:40+00:00 Bissan Ahmed ahmed.bissan1969@gmail.com Andrew Stempel info@cosmosscholars.com Trevor J. McFarland info@cosmosscholars.com Bruce Ksander info@cosmosscholars.com Binoy Appukuttan info@cosmosscholars.com Tim Stout info@cosmosscholars.com <p class="04-abstract"><em>Purpose: </em>Transcriptional enhancer factor 1-related (RTEF-1) also known as TEAD4 is expressed in ocular vascular endothelial cells and plays a role in the control of VEGF expression. Alternative processing of TEAD4 hnRNA results in different proteins able to stimulate or inhibit VEGF gene transcription. The purpose of this study is to test whether short peptide fragments (STY-RMR), representing functional domains of the inhibitory TEAD4<sub>216</sub> isoform, can inhibit tumor as well as endothelial cell proliferation.</p><p class="04-abstract"><em>Experimental Design: </em>Cell proliferation was assessed using a colorimetric assay in cell lines incubated with STY-RMR, the amount of secreted VEGF within media was determined both in treated and control cell lines.</p><p class="04-abstract"><em>Results: </em>Significant dose dependent inhibition of cell proliferation was observed. Maximal inhibition of ocular melanoma (Mel 202 and Mel 207) cell proliferation was observed at a dose of 30 mg/100ml of STY-RMR (87% and 60% inhibition, respectively). At the same dose, more than 50% inhibition was observed in retinoblastoma and breast cancer cells (P &lt;0.001). Significant inhibition of primate ocular endothelial cell proliferation (42% at 30 mg/100 ml (p &lt; 0.001), and retinal pigment epithelial cells showed also a 75% inhibition (p = 0.007). Secreted VEGF was decreased in the media of all tested cell lines that had been exposed to STY-RMR.</p><p class="04-abstract"><em>Conclusion: </em>Functional short peptide domains derived from the TEAD4<sub>216 </sub>isoform may prove to be useful for treatment of ocular tumors and other VEGF dependent neovascular disease.</p><p class="04-abstract">Inhibition of proliferation and VEGF production within ocular endothelial cells indicate the potential of this agent to treat age-related macular degeneration (ARMD) and diabetic retinopathy (DR).</p> 2018-10-31T00:00:00+00:00 Copyright (c)