Effect of Passive Transfer of Anti-Hydatid Cyst Antigen Antisera on Melanoma Tumor Growth in Animal Model

Authors

  • Fereshteah Jafaei Nodeh Isfahan University of Medical Sciences
  • Seyedeh Maryam Sharafi Isfahan University of Medical Sciences
  • Sepideh Tuloee Isfahan University of Medical Sciences
  • Seyed Hossein Hejazi Isfahan University of Medical Sciences
  • Zahra Dorosti Isfahan University of Medical Sciences
  • Hossein Yousofi Darani Isfahan University of Medical Sciences

Keywords:

Antiserum, Tumor, Antigens, Hydatid cyst, Passive immunization.

Abstract

Introduction: Hydatid cyst is the larval stage of Echinococcusgranulosus, a parasite responsible for hydatid disease in human and livestock. The Effect of different antigens of this parasite in preventing the growth of tumor cells has been demonstrated in various studies. Therefore, in this work the effect of passive transfer of antisera raised against different antigens of Hydatid cyst on melanoma cancer growth in animal model has been investigated.

Methods: In this experimental study, antisera against different antigens of hydatid cyst raised in rabbits. C57/black mice were injected with melanoma cells and then they received anti hydatid cyst antigen antisera. Control mice received normal rabbit serum or saline. Tumor size in the case and control groups was measured. Then, the data were analyzed using SPSS software and one-way Anova test.

Findings: The mean tumor area in mice that received antisera against hydatidcyst fluid, protoscolices crude antigen, excretory-secretory antigens of protoscolices and cuticular layer was not significantly different from tumor area of control mice.

Conclusion: The results of this study showed that injection of antisera against antigens of hydatid cyst had no significant effect on melanoma tumor growth. So it is recommended that effect of transfer of immune cells is investigated in future studies.

Author Biographies

Fereshteah Jafaei Nodeh, Isfahan University of Medical Sciences

Medical Parasitology and Mycology

Seyedeh Maryam Sharafi, Isfahan University of Medical Sciences

Infectious Disease and Tropical medicine Research Center

Sepideh Tuloee, Isfahan University of Medical Sciences

Medical Parasitology and Mycology

Seyed Hossein Hejazi, Isfahan University of Medical Sciences

Medical Parasitology and Mycology

Zahra Dorosti, Isfahan University of Medical Sciences

Medical Parasitology and Mycology

Hossein Yousofi Darani, Isfahan University of Medical Sciences

Cancer research prevention research center

Downloads

Published

2015-03-17

Issue

Vol. 2 (2015)

Section

Articles

License

Policy for Journals/Articles with Open Access

Authors who publish with this journal agree to the following terms:
    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

  1. Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work

Policy for Journals / Manuscript with Paid Access

Authors who publish with this journal agree to the following terms:
    1. Publisher retain copyright .

  1. Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work .