Role Of In- Situ Rutin Flavonoid Consisted Myristica Fragrans Silver Nano Particles (MF-Ag-Nps) Against Escherichia Coli and Bacillus Subtilis

Abstract

Most microbiological growth inhibitors are toxic and chemical solvents are harmful for human health. Copper, silver, and gold metallic nanoparticles have several uses in biotechnology and medicinal research. MF-Ag-NPs(silver nanoparticles)are potent anti-inflammatory, anti-proliferative, and anti-microbial agents. The purpose of this study was to biogenically generate silver nanoparticles by extracting rutin from nutmeg, a seed from the Myristica fragrans plant. In order to create Myristica fragrans MF-Ag-NPs, silver nitrate was dissolved in the extract. The transformation of the colour from dark brown to pale brown confirmed the conversion of Ag+ to Ag°. FTIR, EDX, and SEM were used to describe them. Images from scanning electron microscopy demonstrate the presence of silver nanoparticles between 20 and 300 nm in size. The presence of oxygen and silver, as well as the metal's oxidation state, were confirmed by EDAX analysis. The nanoparticles' distinctive functional groups were discovered using FTIR spectroscopy. The antibacterial activity of Myristica fragrans seed extract was assessed. The extract from the seeds of Myrsitica fragrans exhibited the strongest antibacterial properties. These findings imply that antibacterial MF-Ag-NPs phyto-formulated with nutmeg extracts might be used to treat microbial infections in the future.