Evaluation Of Bioactive Compounds in Desmidorchis Indica Stem Extract Using Spectroscopic and Chromatographic Techniques

Abstract

Desmidorchis indica is a small fleshy herbs and umbels terminal belonging to the Apocynaceae family. Extraction is the first step of any medicinal plant study, plays a significant and crucial role on the final result and outcome. In the present study to investigate the qualitative analysis of different extracts (aqueous, ethanol, hexane, hydro-ethanolic and petroleum ether) of Desmidorchis indica stem. Among the various extracts, the hydro-ethanolic extract of Desmidorchis indica stem contains a higher concentration of phytochemicals than other extracts and is used for subsequent studies. The UV-VIS spectroscopy revealed that the characteristic peak indicates the presence of various phytochemicals in the extract. The results of FTIR analysis showed the presence of alcohol, phenol, alkynes, alkenes, aromatic, carboxylic acid, aromatic and aliphatic amines groups in Desmidorchis indica stem extract. HPLC analysis of the hydro-ethanolic extract of Desmidorchis indica stem revealed that the presence of kaempferol, quercetin, epigallocatechin and hypersoide. Thirty compounds were identified in extract of Desmidorchis indica stem by GC-MS analysis. The prevailing compounds are n-hexadecanoic acid, 1-octadecanol, cis-11-eicosenoic acid, heptadecanoic acid, oleic acid, octadecanoic acid, 12,15-octadecadiynoic acid, methyl, 9-octadecenoic acid, eicosanoic acid, 2-methyl-z,z-3,13-octadecadienol, di-(9-octadecenoyl)-glycerol, hexadecanoic acid, 2,3-dihydroxypropyl ester and docosanoic acidwere found in this Desmidorchis indica stem. Overall, it can be concluded that Desmidorchis indica stem is a rich source of phytochemicals confirmed through qualitative, quantitative, spectroscopic and chromatographic techniques.