The Effectiveness of Different Types of PDA Culture Media on the Mycelial Growth of Shimeji Mushroom

Abstract

This experiment aims to compare different types of PDA culture media recipes, including potato, sweet potato, pumpkin, and sugar banana, for their effects on the growth of Shimeji mushroom mycelium. It also aims to provide alternative options for formulating culture media for Shimeji mushroom spawn cultivation. A completely randomized design (CRD) was employed for the experiment, with four treatments and five replicates each, as follows: (T1) potato-based media; (T2) sweet potato-based media; (T3) pumpkin-based media; (T4) sugar banana-based media. Data collection involved daily measurements of the length (in centimeters) and width (in centimeters) for a period of seven days. After the experiment concluded, data analysis was conducted to determine the variation and compare the mean values of the treatments using Duncan's Multiple Range Test (DMRT) and package software. The experimental results showed that over a period of 3, 5, and 7 days, the potato-based media (T1) exhibited the highest growth of Shimeji mushroom mycelium and the highest mycelium density values, measuring 6.2000, 3.2000, and 0.0000a (density scale +++). The pumpkin-based media (T3) had lower mean values of 0.9000, 3.1600, and 0.0000a (density scale +) for mycelium growth and density. Therefore, it can be concluded that among the four PDA media recipes tested over a period of 3, 5, and 7 days, the potato-based media (T1) demonstrated the highest mycelium growth and density values of approximately 6.2000, 3.2000, and 0.0000a  (density scale +++).