Changes in the Cell Surface Markers During Normal Hematopoiesis: A Guide to Cell Isolation
DOI:
https://doi.org/10.15379/2408-9877.2014.01.01.4Keywords:
Cell surface antigen markers, Hematopoiesis, Progenitor cell, Stem cell.Abstract
Hematopoiesis, the process of hematopoietic cell production, largely takes place in the bone marrow (BM) of humans. This process follows a stepwise manner in which hematopoietic stem cells give rise to progenitor cells and they develop the terminally differentiated cells along each lineage through a sequential series of stages. Consequently, constant changes would occur in the gene expressions leading to morphological or functional changes necessary for different stages of maturation. These changes provide us with guides to differentiate different subsets of hematopoietic hierarchy based on the cell surface antigen markers and will help us to isolate various cells from the hematopoietic hierarchy. Here we have a short review on the changes of these surface markers during different stages of development and we have applied an algorithmic approach for the isolation of all these cells based on our current understandings of this system.
References
Payne KJ, Crooks GM. Human hematopoietic lineage commitment. Immunol Rev 2002; 187: 48-64.
Wood B. Multicolor immunophenotyping: human immune system hematopoiesis. Methods Cell Biol. 2004; 75: 559-76.
Rizvi AZ, Wong MH. Epithelial stem cells and their niche: there’s no place like home. Stem Cells 2005; 23: 150-65.
Bissell MJ, Labarge MA. Context, tissue plasticity, and cancer: are cancer stem cells also regulated by the microenvironment? Cancer Cell 2005; 7: 17-23.
Attar A, Khosravi Maharlooi M, Khoshkhou S, Hosseini A, Jaberipour M, Dehghan A, et al. Colony forming unit endothelial cells do not exhibit telomerase alternative splicing variants and activity. Iran Biomed J 2013; 17: 146-151.
National Institute of Health. Stem Cells: Scientific Progress and Future Research Directions. University Press of the Pacific Honolulu, Hawaii; 2001.
Giebel B, Punzel M. Lineage development of hematopoietic stem and progenitor cells. Biol Chem 2008; 389: 813-24.
Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH. Antigenic analysis of hematopoiesis: III. a hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol 1984; 133: 157-165.
Berenson RJ, Andrews RG, Bensinger WI, Kalamasz D, Knitter G, Buckner CD, et al. Antigen CD34+ marrow cells engraft lethally irradiated baboons. J Clin Invest. 1988; 81: 951-955.
Berenson RJ, Bensinger WI, Hill R, Andrews RG, Garcia-Lopez J, Kalamasz DF, et al. Stem cell selection-clinical experience. Prog Clin Biol Res 1990; 333: 403-410; discussion 411-413.
Terstappen LW, Huang S, Safford M, Lansdorp PM, Loken MR. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells. Blood 1991; 77: 1218-1227.
Hao QL, Thiemann FT, Petersen D, Smogorzewska EM, Crooks GM. Extended long-term culture reveals a highly quiescent and primitive human hematopoietic progenitor population. Blood 1996; 88: 3306-3313.
Larochelle A, Vormoor J, Hanenberg H, Wang JC, Bhatia M, Lapidot T, et al. Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: implications for gene therapy. Nat Med 1996; 2: 1329-1337.
Weissman IL, Shizuru JA. The origins of the identification and isolation of hematopoietic stem cells, and their capability to induce donor-specific transplantation tolerance and treat autoimmune diseases. Blood 2008; 112: 3543-3553
Osawa M, Hanada K, Hamada H, Nakauchi H. Long-term lymphohematopoietic reconstitution by a single CD34 low/negative hematopoietic stem cell. Science 1996; 73: 242-245.
Randall TD, Lund FE, Howard MC, Weissman IL. Expression of murine CD38 defines a population of long-term reconstituting hematopoietic stem cells. Blood 1996; 87: 4057-4067.
Liu H, Verfaillie CM.. Myeloid-lymphoid initiating cells (ML-IC) are highly enriched in the rhodamine-ckit+CD33-CD38- fraction of umbilical cord CD34+ cells. Exp Hematol 2002; 30: 582-589.
Verfaillie C, Blakolmer K, McGlave P. Purified primitive human hematopoietic progenitor cells with longterm in vitro repopulating capacity adhere selectively to irradiated bone marrow stroma. J Exp Med 1990; 172: 502-509.
McKenzie JL, Takenaka K, Gan OI, Doedens M, Dick JE. Low rhodamine 123 retention identifies long-term human hematopoietic stem cells within the Lin-CD34+CD38- population. Blood 2007; 109: 543-545.
Hess DA, Wirthlin L, Craft TP, Herrbrich PE, Hohm SA, Lahey R, et al. Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells. Blood 2006; 107: 2162-2169.
Yin AH, Miraglia S, Zanjani ED, Almeida-Porada G, Ogawa M, Leary AG, et al. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 1997; 90: 5002-5012.
de Wynter EA, Buck D, Hart C, Heywood R, Coutinho LH, Clayton A, et al.. CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors. Stem Cells 1998; 16: 387-396.
Giebel B, Corbeil D, Beckmann J, Hohn J, Freund D, Giesen K, et al. Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells. Blood 2004; 104: 2332-2338.
Bhatia M, Bonnet D, Murdoch B, Gan OI, Dick JE. A newly discovered class of human hematopoietic cells with SCID-repopulating activity. Nat Med 1998; 4: 1038- 1045.
Zanjani ED, Almeida-Porada G, Livingston AG, Flake AW, Ogawa M. Human bone marrow CD34- cells engraft in vivo and undergo multilineage expression that includes giving rise to CD34+ cells. Exp Hematol 1998; 26: 353-360.
Gallacher L, Murdoch B, Wu DM, Karanu FN, Keeney M, Bhatia M. Isolation and characterization of human CD34-Lin- and CD34+Lin- hematopoietic stem cells using cell surface markers AC133 and CD7. Blood 2000; 95: 2813-2820.
Manz MG, Miyamoto T, Akashi K, Weissman IL. Prospective isolation of human clonogenic common myeloid progenitors. Proc Natl Acad Sci U S A 2002; 99: 11872-11877.
Galy A, Travis M, Cen D, Chen B. Human T, B, natural killer, and dendritic cells arise from a common bone marrow progenitor cell subset. Immunity 1995; 3: 459-473.
Moore MAS. The hematopoietic system and hematopoiesis. In Neoplastic Hematopathology (Knowels, D. M., Ed.), 2nd ed., pp. 1-42, Lippincott Williams & Wilkins, Philadelphia, PA; 2001.
Shah VO, Civin CI, Loken MR. Flow cytometric analysis of human bone marrow. IV. Differential quantitative expression of T-200 common leukocyte antigen during normal hemopoiesis. J Immunol 1988; 140: 1861-1867.
Terstappen LW, Safford M, Loken MR. Flow cytometric analysis of human bone marrow. III. Neutrophil maturation. Leukemia 1990; 4: 657-663.
Terstappen LW, Loken MR. Myeloid cell differentiation in normal marrow and acute leukemia assessed by multi-dimensional flow cytometry. Anal Cell Pathol 1990; 2: 229-240.
Clarke JL, Watkins W. Alpha1,3-L-fucosyltransferaseexpression in developing human myeloid cells. J Biol Chem 1996; 271: 10317-28.
Skacel PO, Edwards AJ, Harrison CT, Watkins WM. Enzymatic control of the expression of the X determinant (CD15) in human myeloid cells during maturation: The regulatory role of 6-sialytransferase. Blood 1991; 78: 1452-1460.
Elghetany MT. Surface Antigen Changes during Normal Neutrophilic Development: A Critical Review. Blood Cells Mol Dis 2002; 28: 260-74.
Terstappen LW, Buescher S, Nguyen M, Reading C. Differentiation and maturation of growth factor expanded human hematopoietic progenitors assessed by multidimensional flow cytometry. Leukemia 1992; 6: 1001-1010.
De Jong MO, Wagemaker G, Wognum AW. Separation of myeloid and erythroid progenitors based on expression of CD34 and c-kit. Blood 1995; 86: 4076-4085.
Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow: I. Normal erythroid development. Blood 1987; 69: 255-263.
Rogers CE, Bradley MS, Palsson BO, Koller MR. Flow cytometric analysis of human bone marrow perfusion cultures: Erythroid development and relationship with burst-forming units-erythroid. Exp Hematol 1996; 24: 597-604.
Scicchitano MS, McFarland DC, Tierney LA, Narayanan PK, Schwartz LW. In vitro expansion of human cord blood CD36+ erythroid progenitors: Temporal changes in gene and protein expression. Exp Hematol 2003; 31: 760-769.
Debili N, Issaad C, Masse JM, Guichard J, Katz A, Breton-Gorius J, et al. Expression of CD34 and platelet glycoproteins during human megakaryocytic differentiation. Blood 1992; 80: 3022-3035.
Chang Y, Bluteau D, Debili N, Vainchenker W. From hematopoietic stem cells to platelets. J Thromb Haemost 2007; 1: 318-327.
Haddad R, Guardiola P, Izac B, Thibault C, Radich J, Delezoide AL, et al. Molecular characterization of early human T/NK and B-lymphoid progenitor cells in umbilical cord blood. Blood 2004; 104: 3918-3926.
Reynaud D, Lefort N, Manie E, Coulombel L, Levy Y. In vitro identification of human pro-B cells that give rise to macrophages, natural killer cells, and T cells. Blood 2003; 101: 4313-4321.
Hou YH, Srour EF, Ramsey H, Dahl R, Broxmeyer HE, Hromas R. Identification of a human B-cell/myeloid common progenitor by the absence of CXCR4. Blood 2005; 105: 3488-3492.
Akashi K, Traver D, Miyamoto T, Weissman IL. A clonogenic common myeloid progenitor that gives rise to all myeloid lineages. Nature 2000; 404: 193-197.
Katsura Y. Redefinition of lymphoid progenitors. Nature Rev Immunol 2002; 2: 127-132.
Lai AY, Kondo M. Asymmetrical lymphoid and myeloid lineage commitment in multipotent hematopoietic progenitors. J Exp Med 2006; 203: 1867-1873.
Ye M, Graf T. Early decisions in lymphoid development. Curr Opin Immunol 2007; 19: 123-128
Warren LA, Rothenberg EV. Regulatory coding of lymphoid lineage choice by hematopoietic transcription factors. Curr Opin Immunol 2003; 15: 166-175.
Ceredig R, Rolink AG, Brown G. Models of haematopoiesis: seeing the wood for the trees. Nat Rev Immunol 2009; 9: 293-300.
Downloads
Published
Issue
Section
License
Policy for Journals/Articles with Open Access
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work
Policy for Journals / Manuscript with Paid Access
Authors who publish with this journal agree to the following terms:- Publisher retain copyright .
- Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work .