Preanalytical Factors Affecting the Mean Platelet Volume: a Review

Authors

  • Katalin S Zsóri Central Pharmacy, Erzsebet Hospital, Sátoraljaujhely, Hungary
  • Judit M Mahalek Central Intensive Care Unit, Medical Center, Hungarian Defense Forces, Budapest, Hungary
  • Mohammad A Mokarrami Department of Surgery, Bács-Kiskun Hospital, Kalocsa, Hungary
  • Zoltán Csiki Department of medicine, Debrecen university, Debrecen, Hungary
  • Amir H Shemirani MTA-DE Vascular Biology, Thrombosis and Hemostasis Research Group, Hungarian Academy of Sciences, Debrecen, Hungary

DOI:

https://doi.org/10.15379/2408-9877.2016.03.01.05

Keywords:

Mean platelet volume, Preanalytical variables, Hematology analyzer, Anticoagulation, Blood.

Abstract

Mean platelet volume (MPV) as an indicator of platelet activation is a subject of many studies. Since the introduction of automated hematology analyzers, many authors described mean platelet volume as a marker for different pathologic conditions. It is also known that numerous preanalytical conditions could affect MPV results. Specimens are usually tested several hours after blood collection. There are some specific hematology analyzers which are in use by the majority of hematology laboratories. This review demonstrates some important aspects related to MPV measurement in routine laboratory.

References

Martin JF, Trowbridge EA, Salmon G, Plumb J. The biological significance of platelet volume: its relationship to bleeding time, platelet thromboxane B2 production and megakaryocyte nuclear DNA concentration. Thromb Res 1983; 32: 443-60. http://dx.doi.org/10.1016/0049-3848(83)90255-4

Thompson CB, Eaton KA, Princiotta SM, Rushin CA, Valeri CR. Size dependent platelet subpopulations: relationship of platelet volume to ultrastructure, enzymatic activity, and function. Br J Haematol 1982; 50: 509-19. http://dx.doi.org/10.1111/j.1365-2141.1982.tb01947.x

Yavuz S, Ece A. Mean platelet volume as an indicator of disease activity in juvenile SLE. Clin Rheumatol 2014; 33: 637-41. http://dx.doi.org/10.1007/s10067-014-2540-3

Ulutas KT, Dokuyucu R, Sefil F, Yengil E, Sumbul AT, Rizaoglu H, et al. Evaluation of mean platelet volume in patients with type 2 diabetes mellitus and blood glucose regulation: a marker for atherosclerosis? Int J Clin Exp Med 2014; 7: 955-61.

Sansanayudh N, Anothaisintawee T, Muntham D, McEvoy M, Attia J, Thakkinstian A. Mean platelet volume and coronary artery disease: a systematic review and meta-analysis. Int J Cardiol 2014; 175: 433-40. http://dx.doi.org/10.1016/j.ijcard.2014.06.028

Latger-Cannard V, Hoarau M, Salignac S, Baumgart D, Nurden P, Lecompte T. Mean platelet volume: comparison of three analysers towards standardization of platelet morphological phenotype. Int J Lab Hematol 2012; 34: 300-10. http://dx.doi.org/10.1111/j.1751-553X.2011.01396.x

Michelson AD: Platelets, 3rd edn. London; Waltham, MA: Academic Press; 2013.

Briggs C, Harrison P, Machin SJ. Continuing developments with the automated platelet count. Int J Lab Hematol 2007; 29: 77-91. http://dx.doi.org/10.1111/j.1751-553X.2007.00909.x

Briggs C, Harrison P, Grant D, Staves J, MacHin SJ. New quantitative parameters on a recently introduced automated blood cell counter--the XE 2100. Clin Lab Haematol 2000; 22: 345-50. http://dx.doi.org/10.1046/j.1365-2257.2000.00330.x

Trowbridge EA, Reardon DM, Hutchinson D, Pickering C. The routine measurement of platelet volume: a comparison of light-scattering and aperture-impedance technologies. Clin Phys Physiol Meas 1985; 6: 221-38. http://dx.doi.org/10.1088/0143-0815/6/3/003

Macey M, Azam U, McCarthy D, Webb L, Chapman ES, Okrongly D, et al. Evaluation of the anticoagulants EDTA and citrate, theophylline, adenosine, and dipyridamole (CTAD) for assessing platelet activation on the ADVIA 120 hematology system. Clin Chem 2002; 48: 891-9.

Schneider DJ, Tracy PB, Mann KG, Sobel BE. Differential effects of anticoagulants on the activation of platelets ex vivo. Circulation 1997; 96: 2877-83. http://dx.doi.org/10.1161/01.CIR.96.9.2877

Narayanan S. The preanalytic phase. An important component of laboratory medicine. Am J Clin Pathol 2000; 113: 429-52. http://dx.doi.org/10.1309/C0NM-Q7R0-LL2E-B3UY

Thompson CB, Diaz DD, Quinn PG, Lapins M, Kurtz SR, Valeri CR. The role of anticoagulation in the measurement of platelet volumes. Am J Clin Pathol 1983; 80: 327-32.

O'Malley T, Ludlam CA, Fox KA, Elton RA. Measurement of platelet volume using a variety of different anticoagulant and antiplatelet mixtures. Blood Coagul Fibrinolysis 1996; 7: 431-6. http://dx.doi.org/10.1097/00001721-199606000-00001

Dastjerdi MS, Emami T, Najafian A, Amini M. Mean platelet volume measurement, EDTA or citrate? Hematology 2006; 11: 317-9. http://dx.doi.org/10.1080/10245330600954163

Freise KJ, Schmidt RL, Gingerich EL, Veng-Pedersen P, Widness JA. The effect of anticoagulant, storage temperature and dilution on cord blood hematology parameters over time. Int J Lab Hematol 2009; 31: 496-504. http://dx.doi.org/10.1111/j.1751-553X.2008.01066.x

Lance MD, van Oerle R, Henskens YM, Marcus MA. Do we need time adjusted mean platelet volume measurements? Lab Hematol 2010; 16: 28-31. http://dx.doi.org/10.1532/LH96.10011

Diaz-Ricart M, Brunso L, Pino M, Navalon F, Jou JM, Heras M, et al. Preanalytical treatment of EDTA-anticoagulated blood to ensure stabilization of the mean platelet volume and component measured with the ADVIA counters. Thromb Res 2010; 126: e30-5. http://dx.doi.org/10.1016/j.thromres.2010.04.002

McShine RL, Sibinga S, Brozovic B. Differences between the effects of EDTA and citrate anticoagulants on platelet count and mean platelet volume. Clin Lab Haematol 1990; 12: 277-85. http://dx.doi.org/10.1111/j.1365-2257.1990.tb00038.x

McShine RL, Das PC, Sibinga CT, Brozovic B. Effect of EDTA on platelet count and other platelet parameters in blood and blood components collected with CPDA-1. Vox Sang 1991; 61: 84-9. http://dx.doi.org/10.1111/j.1423-0410.1991.tb00251.x

Bath PM. The routine measurement of platelet size using sodium citrate alone as the anticoagulant. Thromb Haemost 1993; 70: 687-90.

Hedberg P, Lehto T. Aging stability of complete blood count and white blood cell differential parameters analyzed by Abbott CELL-DYN Sapphire hematology analyzer. Int J Lab Hematol 2009; 31: 87-96. http://dx.doi.org/10.1111/j.1751-553X.2007.01009.x

Reardon DM, Hutchinson D, Preston FE, Trowbridge EA. The routine measurement of platelet volume: a comparison of aperture-impedance and flow cytometric systems. Clin Lab Haematol 1985; 7: 251-7. http://dx.doi.org/10.1111/j.1365-2257.1985.tb00033.x

Recommendations of the International Council for Standardization in Haematology for Ethylenediaminetetraacetic Acid Anticoagulation of Blood for Blood Cell Counting and Sizing. International Council for Standardization in Haematology: Expert Panel on Cytometry. Am J Clin Pathol 1993; 100: 371-2.

Daves M, Zagler EM, Cemin R, Gnech F, Joos A, Platzgummer S, et al. Sample stability for complete blood cell count using the Sysmex XN haematological analyser. Blood Transfus 2015; 13: 576-82.

White JG. Effects of ethylenediamine tetracetic acid (EDTA) on platelet structure. Scand J Haematol 1968; 5: 241-54. http://dx.doi.org/10.1111/j.1600-0609.1968.tb01743.x

Gachet C, Hanau D, Spehner D, Brisson C, Garaud JC, Schmitt DA, et al. Alpha IIb beta 3 integrin dissociation induced by EDTA results in morphological changes of the platelet surface-connected canalicular system with differential location of the two separate subunits. J Cell Biol 1993; 120: 1021-30. http://dx.doi.org/10.1083/jcb.120.4.1021

Shreiner DP, Bell WR. Pseudothrombocytopenia: manifestation of a new type of platelet agglutinin. Blood 1973; 42: 541-9.

Schrezenmeier H, Muller H, Gunsilius E, Heimpel H, Seifried E. Anticoagulant-induced pseudothrombocytopenia and pseudoleucocytosis. Thromb Haemost 1995; 73: 506-13.

Schuff-Werner P, Steiner M, Fenger S, Gross HJ, Bierlich A, Dreissiger K, et al. Effective estimation of correct platelet counts in pseudothrombocytopenia using an alternative anticoagulant based on magnesium salt. Br J Haematol 2013; 162: 684-92. http://dx.doi.org/10.1111/bjh.12443

Manthorpe R, Kofod B, Wiik A, Saxtrup O, Svehag SE. Pseudothrombocytopenia. In vitro studies on the underlying mechanism. Scand J Haematol 1981; 26: 385-92. http://dx.doi.org/10.1111/j.1600-0609.1981.tb01679.x

Savage RA. Pseudoleukocytosis due to EDTA-induced platelet clumping. Am J Clin Pathol 1984; 81: 317-22.

Kolesnikova IV, Potapov SV, Yurkin MA, Hoekstra AG, Maltsev VP, Semyanov KA. Determination of volume, shape and refractive index of individual blood platelets. J Quant Spectrosc Radiat Transf 2006; 102: 37-45. http://dx.doi.org/10.1016/j.jqsrt.2006.02.050

Lance MD. Mean platelet volume needs standardisation. Comment on Asher et al. Thromb Haemost 2014; 112: 137-141. Thromb Haemost 2015; 113: 908. http://dx.doi.org/10.1160/TH14-07-0590

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Published

2016-01-20

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Vol. 3 No. 1 (2016)

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