Expression of the Recombinant Soluble Glycoprotein IIb: Lessons Learned and Future Directions

Authors

  • Younis Skaik Department of Laboratory Medicine, Faculty of Applied Medical Sciences, AL-Azhar University-Gaza, Palestine

DOI:

https://doi.org/10.15379/2408-9877.2015.02.02.03

Keywords:

GPIIb, Soluble GPIIb, Recombinant GPIIb, Retention signal.

Abstract

The platelet glycoprotein IIb (GPIIb) is an integral membrane protein, which forms with GPIIIa a calcium-dependent heterodimer. This heterodimer forms in the endoplasmic reticulum (RE) and in the absence of heterodimer formation, both GPIIb and GPIIIa are retained and degraded in the ER. Efforts have been previously made to produce recombinantly the soluble GPIIb, which can be used in the solid phase assays for the antibody identification. However, all previous attempts to produce such a construct have not yield any product. This mini-review describes the previous attempts and the future challenges.

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Published

2015-07-30

Issue

Vol. 2 No. 2 (2015)

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