|International Journal of Membrane Science and Technology (Volume 2 Issue 1)|
|Discrimination of Conductance of Lower and Higher Oligomeric Alamethicin Pores|
Dinara Aliverdieva and Dmitry Mamaev
Published: 15 May 2015
|Experimental studies of antimicrobial peptides having a pore-forming mechanism of action have recently attracted increasing interest due to their broad spectrum of activity and numerous clinical applications. Alamethicin has been widely studied as an antimicrobial peptide and a model for ion channel-forming proteins. However, the lack of simple analytical tools for studying mechanisms of interaction of pore-forming peptides and biological membranes at very low peptide concentrations remains a problem. Here in a short report, we describe an experimental approach for performing time-dependent discrimination of transmembrane current induced by fractions of lower- and higher-order oligomeric alamethicin pores using mitochondria.|
Alamethicin, pore formation, potassium transmembrane current, rat liver mitochondria.